Prof. Karsten Haupt

Karsten Haupt is a Biochemist from the University of Leipzig. After obtaining a PhD from Université de Technologie de Compiègne (UTC), he was a researcher at Lund University, Sweden, at INSERM, Paris, and at University of Paris 12. Since 2003 he has been a full professor of Bioengineering at UTC, and since 2012 the Head of the CNRS Laboratory for Enzyme and Cell Engineering. He is a Senior Member of Institut Universitaire de France. His main research interests include affinity technology, chemical sensors, synthetic antibodies, and nanomaterials for biomedicine.

Relevant publication: K. Haupt, P.X. Medina Rangel, B. Tse Sum Bui (2020) Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy. Chem. Rev. 120, 9554-9582.

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Abstract

Molecularly imprinted polymers (MIPs) [1] are synthetic antibodies that specifically recognize molecular targets. They are cross-linked polymers synthesized in the presence of a molecular template, which induces three-dimensional binding sites in the polymer that are complementary to the template in size, shape and chemical functionality. MIPs against proteins are obtained through a rational approach starting with in silico epitope design, similar to the generation of peptide antibodies. Chemically synthesized peptide epitopes can then be used as templates in a solid-phase protocol for MIP synthesis [2,3]. Binding assays using SPR or fluorescence, solution STD NMR and WaterLOGSY studies, as well as single molecule fluorescence methods, allow to confirm the existence of specific binding sites. We demonstrate the potential of MIP nanogels (~50 nm) for medical diagnostics [3], bioimaging [4] and medical therapy [4,5], on the example of cell surface protein targets [4], as well as soluble cytokines and biomarkers [3,5].

References

[1] K. Haupt, P.X. Medina Rangel, B. Tse Sum Bui, Chem. Rev. 120 (2020) 9554-9582.
[2] B. Tse Sum Bui, A. Mier, K. Haupt, Small 19 (2023) 2206453
[3] A. Mier, et al., Angew. Chem. Int. Ed. 60 (2021) 20849-20857.
[4] P.X. Medina Rangel et al., Angew. Chem. Int. Ed. 59 (2020) 2816-2822.
[5] C. Herrera León, et al., Angew. Chem. Int. Ed. 62 (2023) e202306274.
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